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OBJECTIVE:To investigate effect of drug-containing serum of Xinfeng capsules on myocardial cell growth.METHODS:Drug-containing serum of Xinfeng capsules rat models were established by intragastricly administrated Xinfeng capsules.MTT assay was used to evaluated H9C2 cells viability.Hgc2 cells were divided into-normal control group,triptolide group,lipopolysaccharide (LPS) group,drug-containing serum group and miRNA-21 inhibitor group.microRNA-21 (miRNA-21) inhibitor was structured and transfected into H9C2 cells.West blot and immunofluorescence assay were applied to examine toll-like receptor 4 (TLR4),phosphorylated p-38 (p-p38) and p-p65 expression.Quantitative real-time PCR (qRT-PCR) was used to evaluated mRNA levels of miRNA-21.Enzyme linked immunosorbent (ELISA) was used to measure tumor necrosis factor o (TNF-α),IL-6 and IL-17 levels.RESULTS:Drug-containing serum treatment significantly increased cell viability compared to LPS treated group.qRT-PCR results indicated that miRNA-21 levels were significantly decreased in drugcontaining serum group compared to LPS group.Early and late apoptosis in drug-containing serum group were significantly decreased compared to LPS group.West blot and immunofluorescence assay results showed that TLR4,p-p38 and p-p65 levels in drug-containing serum group were significantly decreased compared to LPS group.ELISA findings indicated that drug-containing serum significantly decreased inflammatory cytokine levels ofTNF-α,IL-6 and IL-17.CONCLUSION:Drug-containing serum of Xinfeng capsules protect against lipopolysaccharide instructed H9C2 cells from death by enhancing miRNA-21 and inhibiting TLR4/p-p38/p-p65 signaling pathway and proinflammatory cytokines expression.