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本研究用构建的ＣＴＬＡ 4Ｉｇ重组腺病毒转染体外培养的人真核细胞 ,以探讨核载体临床治疗应用的可行性。1 材料 2 93细胞购自ＡＴＣＣ (美国 ) ,鼠抗人ＣＴＬＡ 4Ｉｇ单抗购自Ｐｈａｒｍｉｎｇｅｎ公司 ,5ｍｌ亲合层析柱ＨｉＴｒａｐＰｒｏｔｅｉｎＡ为Ｐｈａｒｍａｃｉ
In this study, the constructed recombinant CTLA 4Ig adenovirus transfection of human eukaryotic cells in vitro to explore the feasibility of clinical application of nuclear vector. 1 Materials 2 93 cells were purchased from ATCC (USA), mouse anti-human CTLA 4Ig monoclonal antibody was purchased from Pharmingen, 5ml affinity column HiTrapProteinA was Pharmaci