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目的探讨在原发性肝癌发展的不同阶段肝糖原的变化规律。方法清洁健康BALB/c雄性小鼠40只,分为正常组(n=3)与模型组(n=37)。将正常组3只小鼠处死,取肝;模型组小鼠每日饲以二乙基亚硝胺(DEN)溶液30μg·m L-1为饮用水,于诱癌4,8,12,16,20,24周,各取3只小鼠进行肝取材(n=21)。将各组肝组织进行糖原过碘酸雪夫氏(PAS)染色。并对样本进行平均光密度分析,用ANOVA显著分析和Student-Newman-Keuls检验。结果正常组和8,12周模型组的糖原含量依次显著降低,16周模型组糖原含量达最低;随后20,24周模型组肝糖原含量依次显著增加。结论 DEN诱导小鼠发生肝癌的过程中,肝糖原的变化规律为先减少后增加。
Objective To investigate the changes of hepatic glycogen at different stages of the development of primary liver cancer. Methods Forty healthy BALB / c male mice were divided into normal group (n = 3) and model group (n = 37). The mice in the normal group were sacrificed and the liver was taken. The mice in the model group were fed with 30μg · m L-1 of diethylnitrosamine (DEN) solution daily for drinking water and 4,8,12,16 At 20 and 24 weeks, three mice were selected for liver harvest (n = 21). Liver tissues of each group were subjected to glycogen periodic acid Schiff (PAS) staining. The samples were subjected to mean optical density analysis, with significant analysis by ANOVA and Student-Newman-Keuls test. Results The levels of glycogen in the normal group, the model group at 8 and 12 weeks were significantly lower than those in the model group at 16 weeks and the lowest at 16 weeks. The levels of glycogen in the model group were significantly increased at 20 and 24 weeks. Conclusion DEN-induced liver cancer in mice during the course of change of glycogen for the first increase and then decreased.