论文部分内容阅读
IFN-r 是一种有效的免疫调节因子,它可以激活NK 细胞和Mφ,在CTL 细胞的诱导、分化和激活中起重要作用。为了能够在体内建立一个能连续提供IFN-r 的场所,以促进CTL 细胞的抑制效应。作者将小鼠的IFN-r cDNA 转染给小鼠神经胶质瘤特异性CTL 克隆(E-4),并获得可连续产生IFN-r 的2个亚株(Er-6,Er-9),转染有IFN-r cDNA 的CTLs 其杀伤活性比转染前可提高2～3倍。作者首先进行了体外细胞毒试验,把1×10~4标记有0.1mCi~(51)Cr 的203-神经胶质瘤细胞(203-G)加到效应
IFN-r is an effective immunomodulatory factor that activates NK cells and Mφ and plays an important role in the induction, differentiation and activation of CTL cells. In order to be able to establish a site in the body that can continuously provide IFN-r, in order to promote the inhibitory effect of CTL cells. The authors transfected mouse IFN-r cDNA into a mouse glioma-specific CTL clone (E-4) and obtained two substrains (Er-6, Er-9) that could produce IFN-r continuously. The killing activity of CTLs transfected with IFN-r cDNA was 2-3 times higher than that before transfection. The authors first performed an in vitro cytotoxicity assay, adding 1×10~4 203-glioma cells (203-G) labeled with 0.1mCi~(51)Cr to the effect.