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目的探讨吉西他滨对人胰腺癌细胞系BxPC-3的细胞毒性、放射增敏作用。方法用MTT法测定吉西他滨对BxPC-3细胞生长的作用,集落形成法观察其对BxPC-3细胞的放射增敏作用,流式细胞术分析吉西他滨作用后细胞周期及凋亡变化,免疫细胞化学染色观察Bcl-2、Bax蛋白表达。结果吉西他滨对BxPC-3细胞具有浓度依赖性抑制作用(IC50=106μg/ml),选择浓度为IC10(0.04μg/ml),可增加X射线对BxPC-3的杀伤作用,放射增敏比SER为1.15。药物浓度为0.04μg/ml时S期细胞比例增高。吉西他滨作用24 h后,细胞Bax表达增加,而Bcl-2表达下降。结论吉西他滨对人胰腺癌BxPC-3细胞系有细胞毒性作用和一定的放射增敏作用;其机制可能与吉西他滨引起S期阻滞和调节凋亡相关基因的表达有关。
Objective To investigate the cytotoxicity and radiosensitization of gemcitabine on human pancreatic cancer cell line BxPC-3. Methods The effect of gemcitabine on the growth of BxPC-3 cells was determined by MTT method. The radiosensitization of BxPC-3 cells was observed by colony formation assay. The cell cycle and apoptosis were detected by flow cytometry. Bcl-2, Bax protein expression was observed. Results Gemcitabine could inhibit the proliferation of BxPC-3 cells in a dose-dependent manner (IC50 = 106μg / ml) and IC10 (0.04μg / ml) 1.15. When the drug concentration was 0.04μg / ml, the proportion of S phase cells increased. Gemcitabine for 24 h, the expression of Bax increased, while the expression of Bcl-2 decreased. Conclusion Gemcitabine has cytotoxicity and radiosensitization effect on human pancreatic cancer BxPC-3 cell line, which may be related to the induction of S phase arrest and the regulation of apoptosis-related genes by gemcitabine.