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为明确抗戊唑醇草莓枯萎病菌Fusarium oxysporum f.sp.fragariae突变体ZY-W的生理生化特性,分析其抗性风险及可能的抗性机制,比较了对戊唑醇抗性达34.2倍的ZY-W和敏感菌株ZY在苯丙氨酸解氨酶(PAL)及过氧化物酶(POD)活力、对渗透压的敏感性、相对渗率、可溶性蛋白含量及酯酶同工酶图谱等方面的差异。结果表明:ZY-W突变菌株体内的POD和PAL活力均高于ZY;经不同浓度药剂处理0～24 h,2菌株PAL活力均呈先上升后下降的趋势,并在处理后1.5 h达到最高值;2菌株的POD活力则一直处于上升状态,24 h时达到最高值,且此时2菌株间POD活力差异最大;仅用清水处理0～24 h,2菌株的PAL和POD活力均一直处于上升状态。在不同处理时间及药剂浓度下,ZY的相对渗率为ZY-W的1.0～2.4倍,表明ZY-W菌株的细胞膜透性低于ZY;ZY-W对渗透压的敏感性及体内可溶性蛋白含量均显著高于ZY,其中ZY-W的可溶性蛋白含量是ZY的1.3倍;在突变菌株ZY-W的酯酶同工酶图谱中发现了Rf值为0.24的特征性谱带。表明草莓枯萎病菌对戊唑醇产生抗性的同时,伴随着其生理生化特性的改变。
In order to clarify the physiological and biochemical characteristics of the Fusarium oxysporum f.sp.fragariae mutant ZY-W, the risk of resistance and the possible mechanism of resistance were analyzed. The resistance to tebuconazole was 34.2-fold The sensitivity of ZY-W and sensitive strain ZY to phenylalanine ammonia lyase (PAL) and peroxidase (POD) activity, osmotic pressure, relative osmolarity, soluble protein content and esterase isozyme The difference. The results showed that the activity of POD and PAL of ZY-W mutant strain was higher than that of ZY. The PAL activity of 2 strain increased first and then decreased after being treated with different concentrations for 0-24 h and reached the highest at 1.5 h POD activity of 2 strains kept rising all the time and reached the highest value at 24 h, and the difference of POD activity between 2 strains at this time was the largest. The activities of PAL and POD of 2 strains were always at 0-24 h Rising state. The relative osmolality of ZY was 1.0 ~ 2.4 times of that of ZY-W under different treatment time and concentration, which indicated that the cell membrane permeability of ZY-W strain was lower than that of ZY; the sensitivity of ZY-W to osmotic pressure and the content of soluble protein The content of soluble protein in ZY-W was 1.3 times that of ZY, and the characteristic band with Rf value of 0.24 was found in the esterase isozyme of ZY-W. The results showed that Fusarium oxysporum f.sp. crispus was resistant to tebuconazole and accompanied with its physiological and biochemical characteristics.