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目的：探讨克服肿瘤细胞多药耐药（ＭＤＲ）的方法，提高化疗效果。方法：采用多药耐药反义基因（ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ）逆转Ｋ５６２／ＡＤＭ肿瘤细胞的ＭＤＲ，而诱导肿瘤细胞凋亡。结果：ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ诱导Ｋ５６２／ＡＤＭ细胞产生大量ＤＮＡ断片，流式细胞仪检测发现几乎全部ｍｄｒ－１＋Ｋ５６２／ＡＤＭ细胞发生凋亡。结论：ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ能有效、特异地抑制ｍｄｒ－１基因表达，逆转肿瘤细胞的ＭＤＲ，促进阿霉素诱导Ｋ５６２／ＡＤＭ细胞凋亡，为其临床应用提供理论依据。
Objective: To explore ways to overcome the multidrug resistance (MDR) of tumor cells and improve the efficacy of chemotherapy. METHODS: Multidrug resistance antisense gene (mdr-1-ASPS-ODN) was used to reverse the MDR of K562/ADM tumor cells and induce tumor cell apoptosis. RESULTS: mdr-1-ASPS-ODN induced large numbers of DNA fragments in K562/ADM cells. Flow cytometry revealed that almost all mdr-1+K562/ADM cells developed apoptosis. Conclusion: mdr-1-ASPS-ODN can effectively and specifically inhibit the expression of mdr-1 gene, reverse the MDR of tumor cells, and promote apoptosis of K562/ADM cells induced by doxorubicin, which provides a theoretical basis for its clinical application.