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试验表明:影响病毒产量最主要的因子是饲毒时寄主幼虫的虫龄,其次是病毒浓度。此外,病毒浓度与虫龄的组合和病毒浓度与饲毒持续时间的组合,也都有显著的影响。在4龄后期采用1.15×10~7颗粒体/ml的浓度,饲毒24小时,得到了最高的产量。 每头病死幼虫的平均颗粒体含量与死亡时的平均体重有极显著的相关性,两者之间的回归系数为0.1016。体重每增加1mg,平均可以增加1.016×10~8颗粒体。 单位体重的颗粒体含量,发黑的病死幼虫>刚死幼虫>将死幼虫>发黑的死预蛹>发黑的死蛹>带毒的活蛹,前四者之间差异均极显著。但由于死后发黑的幼虫表皮脆弱易破,采收困难,且时间长易为杂菌污染,故以刚死即采收为最好。
The experiment shows that the main factor affecting the yield of virus is the larval age of host larvae when feeding, followed by the virus concentration. In addition, the combination of virus concentration and age of insects and the combination of virus concentration and feeding duration also have significant effects. In the late fourth instar using 1.15 × 10 ~ 7 particles / ml concentration, feeding for 24 hours, the highest yield. The average particle content of each dead larvae was significantly correlated with the average body weight at the time of death, with a regression coefficient of 0.1016. An increase of 1mg per body weight, an average increase of 1.016 × 10-8 particles. The body weight of the granule content, black dead larvae> dead larvae> dead larvae> black dead pre-pupa> black dead pupa> infected pupae, the first four differences were significant. However, due to the death of the black larvae vulnerable epidermal fragile, harvesting difficulties, and for a long time easily contaminated by miscellaneous bacteria, so just died or harvested as the best.