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作者以变链菌 Ingbritt 株于含葡萄糖的胰蛋白胨-酵母提取物的培养基里培养。获取的菌体经0.2%甲醛杀死、洗涤、冻干或直接悬浮于含10%聚乙烯吡略酮(PVP),5%谷氨酸钠的溶液中供免疫用。
The authors cultivated the Streptomyces strain Ingbritt in a glucose-containing tryptone-yeast extract medium. The obtained cells were killed by 0.2% formaldehyde, washed, lyophilized or directly suspended in a solution containing 10% polyvinylpyrrolidone (PVP) and 5% sodium glutamate for immunization.