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探讨Ｃａ２＋ＡＴＰ酶在高压氧条件下对微血管作用的机理。方法：实验用Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠，雌雄均用，实验动物随机被分为：正常对照组、常压纯氧组、０．２５ ＭＰａ高压氧组和０．４ ＭＰａ高压氧组。用定磷法测定大鼠血管平滑肌Ｃａ~２＋－ＡＴＰ酶活力。结果：０．１ＭＰａ纯氧和０．２５ ＭＰａ高压氧作用后，Ｃａ~２＋ＡＴＰ酶活力增加，与正常对照组相比，０．１ＭＰａ纯氧组无显著性改变（Ｐ＞０．０５），而０．２５ＭＰａ高压氧组有非常显著的上升（Ｐ＜０．０１）；但０．４ ＭＰａ高压氧组血管平滑肌Ｃａ~２＋－ＡＴＰ酶活力下降，与正常对照组相比有显著性差异（Ｐ＜０．０５）。结论：血管平滑肌细胞Ｃａ~２＋－ＡＴＰ酶活力受高压氧作用的影响，在０．１ＭＰａ纯氧和０．２５ＭＰａ高压氧下可使酶活力增加，但过高压力的高压氧则降低其活力。
To investigate the mechanism of action of Ca2 + ATPase on microvascular in hyperbaric oxygenation. Methods: Experimental Sprague-Dawley rats were used both male and female. The experimental animals were randomly divided into normal control group, normal pressure pure oxygen group, 0.25 MPa hyperbaric oxygen group and 0.4 MPa hyperbaric oxygen group. Determination of Ca ~ (2 +) - ATPase activity in rat vascular smooth muscle by fixed phosphorus method. Results: Compared with normal control group, the activity of Ca ~ (2 +) + ATPase increased after 0.1 MPa pure oxygen and 0.25 MPa hyperbaric oxygen had no significant change (P> 0.05) compared with the normal control group (P <0.01). However, the activity of Ca ~ (2 +) - ATPase in vascular smooth muscle of 0.4 MPa hyperbaric oxygen group decreased significantly compared with the control group (P <0.05). CONCLUSION: The Ca ~ (2 +) - ATPase activity of vascular smooth muscle cells is affected by hyperbaric oxygen. Enzymatic activities are increased under 0.1MPa pure oxygen and 0.25MPa hyperbaric oxygen, but hyperbaric oxygen under high pressure reduces its activity.