[Objective] To determine and compare the contents of plumbagin in the leaves of Plumbago zeylanica L. by different extraction methods, and to select the optimal extraction solvent. [Method] Soxhlet extraction method was adopted; plumbagin in the leaves of P. zeylanica was extracted by different solvents. Its content was detected by HPLC, so as to select the optimal extraction solvent. [Result] Contents of plumbagin extracted by chloroform, petroleum ether (II), 95% ethanol and anhydrous methanol were 0.051 1%, 0.034 1%, 0.023 3% and 0.019 1%, respectively. Content of plumbagin in P. zeylanica extracted by chloroform was the highest. [Conclusion] There were significant differences in the content of plumbagin in P. zeylanica fresh leaves by different extraction solvents. [Objective] To determine and compare the contents of plumbagin in the leaves of Plumbago zeylanica L. by different extraction methods, and to select the optimal extraction solvent. [Method] Soxhlet extraction method was adopted; plumbagin in the leaves of P. zeylanica was [Result] Contents of plumbagin extracted by chloroform, petroleum ether (II), 95% ethanol and anhydrous methanol were 0.051 1%, 0.034 1 %, 0.023 3% and 0.019 1%, respectively. Content of plumbagin in P. zeylanica extracted by chloroform was the highest. [Conclusion] There were significant differences in the content of plumbagin in P. zeylanica fresh leaves by different extraction solvents.