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目的:探讨丹参素和丹参酮ⅡA对肠系膜微循环障碍的影响。方法:将SD大鼠40只,随机分成5组,分别为正常组、模型组、阳性药酚妥拉明组(10 mg·kg-1),丹参素组(30 mg·kg-1)和丹参酮ⅡA组(30 mg·kg-1)。通过向肠系膜滴加去甲肾上腺素(NA)造成局部微循环障碍,一次性经舌下静脉注入药物(丹参素、丹参酮ⅡA和酚妥拉明或者生理盐水),10 min后向将近回盲部的20 cm的肠系膜区域滴加0.05 g·L-1的NA溶液100μL,观察滴加NA后1,3,5,10,20,30 min时的大鼠肠系膜微动脉管径,并记录肠系膜微动脉血液流态镜下血液流态、毛细血管开放率和血流恢复时间。结果:与正常组比较,模型组NA对肠系膜微动脉有明显的收缩作用,血液流态分数明显增加,毛细血管开放率明显降低,血流恢复时间明显升高,均具有明显统计学意义(P<0.01);与模型组比较,丹参素和丹参酮ⅡA能在一定程度减轻NA对肠系膜微动脉的收缩作用,改善血液流态,提高肠系膜毛细血管网交点开放率,缩短微循环障碍的恢复时间(P<0.05,P<0.01)。结论:丹参素和丹参酮ⅡA能改善NA引起的大鼠肠系膜微循环障碍。
Objective: To investigate the effects of Danshensu and tanshinone ⅡA on mesenteric microcirculation. Methods: Forty SD rats were randomly divided into five groups: normal group, model group, positive group (10 mg · kg-1), Danshensu group (30 mg · kg- Tanshinone Ⅱ A group (30 mg · kg-1). Local microcirculation was caused by the addition of norepinephrine (NA) into the mesentery, and the drugs (Danshensu, Tanshinone IIA, and Phentolamine or normal saline) were injected once through the sublingual vein. After 10 min, Of 20 cm of mesenteric area was added dropwise with 0.05 g · L-1 NA solution 100 μL NA was observed after 1, 3, 5, 10, 20, 30 min of rat mesenteric arteriolar diameter, and record mesenteric micro Arterial blood flow microscopy blood flow, capillary open rate and blood flow recovery time. Results: Compared with the normal group, NA in the model group showed significant systolic effect on the mesenteric arterioles, the blood flow fraction increased significantly, the capillary opening rate was significantly decreased, and the recovery time of blood flow was significantly increased (P <0.01). Compared with the model group, Danshensu and tanshinone ⅡA can reduce the contractile effect of NA on the mesenteric arterioles to a certain extent, improve the blood flow state, improve the open rate of mesenteric capillary network intersection and shorten the recovery time of microcirculation disturbance P <0.05, P <0.01). Conclusion: Danshensu and tanshinone Ⅱ A can improve the NA-induced mesenteric microcirculation disorder in rats.