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将正常血小板与用胶原刺激的血小板的胞浆部分与膜部分进行SDS-聚丙酰胺凝胶电泳(PAGE),并测定各部分的肌动蛋白激活的肌球蛋白Mg～(2+)-ATPase活力(AM-Mg～(2+)-ATPase)后发现:正常血小板膜部分所连接的肌球蛋白与肌动蛋白分别占血小板中此两种蛋白总量的26.40％)和31.55％。血小板受胶原刺激后则分别变为87.83％和42.17％。胞浆中的AM-Mg～(2+)-ATPase活力从正常的1.43±1.37nmolPi/min(每毫克肌球蛋白)升高到刺激后的4.66±1.51nmol Pi/min(每毫克肌球蛋白)。这些结果表明;血小板的胶原受体被结合后,有大量肌球蛋白和较少量肌动蛋白连接到胞膜上。另一方面,胞浆中的AM-Mg～(2+)-ATPase活力也明显增加。这些变化可能与血小板的形态变化和功能变化有密切关系。
The cytoplasm and membrane fractions of normal platelets and collagen - stimulated platelets were subjected to SDS - polyacrylamide gel electrophoresis (PAGE) and their actin - activated myosin Mg ~ (2 +) - ATPase activity (AM-Mg ~ (2 +) - ATPase) and found that myosin and actin were respectively accounted for 26.40% and 31.55% of the total amount of these two proteins in platelets. After platelet stimulation by collagen, they became 87.83% and 42.17% respectively. AM-Mg ~ (2 +) - ATPase activity in the cytoplasm rose from normal 1.43 ± 1.37 nmolPi / min (per mg myosin) to 4.66 ± 1.51 nmol Pi / min after stimulation (per mg of myosin ). These results indicate that platelet collagen receptors are bound to a large amount of myosin and a smaller amount of actin to the membrane. On the other hand, the cytosolic AM-Mg ~ (2 +) - ATPase activity also increased significantly. These changes may be related to changes in platelet morphology and functional changes are closely related.