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目的:建立测定参命源皂甙人参锭中5种人参皂苷(Rg1、Re、Rb1、Rb2和Rd)浓度的高效液相色谱(HPLC)法,为工业生产的质量控制提供依据。方法:采用HPLC法测定参命源皂甙人参锭中人参皂苷的浓度,检测条件:Alltima C18(250.0mm×4.6mm,5.0μm)色谱柱,以乙腈为流动相A,0.1%磷酸水溶液为流动相B,梯度洗脱;流速为1.0mL·min-1;检测波长为203nm;柱温为40℃。结果:人参皂苷Rg1、Re、Rb1、Rb2和Rd的线性关系良好,相关系数(r)均大于0.999 1,平均回收率为99.5%~102.6%;精密度实验、稳定性实验和重复性实验检测人参皂苷Rg1、Re、Rb1、Rb2和Rd的相对标准差(RSD)分别为0.35%~0.76%、0.74%~1.44%和0.78%~1.47%。结论:HPLC法检测参命源皂甙人参锭中人参皂苷浓度的方法准确、简单可行、重复性好,可以用于参命源皂甙人参锭的质量控制。
Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of ginsenosides (Rg1, Re, Rb1, Rb2 and Rd) in ginseng ingot of ginseng source saponins, and provide the basis for the quality control of industrial production. Methods: The concentration of ginsenosides in ginseng saponin of ginseng source was determined by HPLC. The detection conditions were as follows: the mobile phase was acetonitrile (A), 0.1% phosphoric acid aqueous solution B, gradient elution; flow rate of 1.0mL · min-1; detection wavelength of 203nm; column temperature of 40 ℃. Results: The linear relationship between ginsenosides Rg1, Re, Rb1, Rb2 and Rd was good, the correlation coefficients (r) were all above 0.999 1, and the average recoveries were between 99.5% and 102.6%. The precision test, stability test and repeatability test The relative standard deviations (RSD) of ginsenosides Rg1, Re, Rb1, Rb2 and Rd were 0.35% ~ 0.76%, 0.74% ~ 1.44% and 0.78% ~ 1.47%, respectively. Conclusion: The method of HPLC for the determination of ginseng saponin in ginseng source saponin is accurate, simple and feasible with good reproducibility and can be used for quality control of ginseng saponin.