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本实验室合成的新光度试剂5-[(4′-二乙氨基苯基)-偶氮]-8-羟基喹啉(简称5-DEPAQ)能与铜、镉、钴、镍、锌等金属离子发生灵敏的显色反应。本文研究该试剂与铜(Ⅱ)的显色反应条件,在吐温-80的增溶作用下,pH为6.5～8.7时形成稳定的1:2(Cu:R)红色配合物,表观摩尔吸光系数ε_(530)=6.19×10~4l·mol~(-1)·cm~(-1),铜含量在0～1.5μg·ml~(-1)范围内符合比耳定律。镉、钴、锰、镍、锌等元素的干扰可采用掩蔽差减光度法消除。试验部分一主要试剂与仪器铜标准溶液:10μg·ml~(-1) 5-DEPAQ溶液:8.0×10~(-4)mol·L~(-1)二甲基甲酰胺溶液
The new photometric reagent 5 - [(4’-diethylaminophenyl) -azo] -8-hydroxyquinoline (5-DEPAQ) synthesized by our laboratory can react with metals such as copper, cadmium, cobalt, nickel and zinc Ion-sensitive color reaction. In this paper, the color reaction conditions between this reagent and copper (Ⅱ) were studied. Under the solubilization of Tween-80, a stable 1: 2 (Cu: R) red complex was formed at pH 6.5 ~ 8.7. The extinction coefficientε_ (530) = 6.19 × 10 ~ 4l · mol ~ (-1) · cm ~ (-1), and Beer’s law was obeyed in the range of0 ~ 1.5μg · ml ~ (-1) Cadmium, cobalt, manganese, nickel, zinc and other elements of the interference can be masked subtractive spectrophotometry eliminate. Experimental part of a major reagents and equipment Copper standard solution: 10μg · ml -1 5-DEPAQ solution: 8.0 × 10 -4 mol·L -1 dimethylformamide solution