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目的:构建人食管鳞状细胞癌组织来源的移植瘤模型,并了解其病理学特征和增殖相关的信号通路活化情况。方法:将人食管癌组织移植于重度联合免疫缺陷(SCID)小鼠皮下,待移植瘤长成后对其进行鼠间连续传代。观察第1、第2、第3代移植瘤的生长特性。并对患者肿瘤组织、第1代和第3代移植瘤进行HE染色和CK5/6、p63、p40免疫组织化学染色分析。Western blot实验检测4例所建立的移植瘤中m TOR、p-m TOR、p70S6K、pp70S6K、Akt1、p-Akt(Ser473)、Erk1/2和p-Erk1/2的表达情况。结果:成功建立移植瘤模型,移植瘤生长稳定并能连续传代。各移植瘤组织病理组织类型和CK5/6、p63、p40表达阳性与患者肿瘤组织一致。而在不同病人来源的移植瘤组织中信号转导通路蛋白的活化程度差异有统计学意义。结论:成功建立了人食管鳞状细胞癌组织来源的食管癌移植瘤模型,初步论证该模型能够反映患者的病理特征。
OBJECTIVE: To construct a transplanted tumor model of human esophageal squamous cell carcinoma tissue and to understand its pathological features and proliferation-related signaling pathway activation. Methods: Human esophageal cancer tissues were transplanted subcutaneously into severe combined immunodeficient (SCID) mice. After transplantation, the mice were passaged continuously. The growth characteristics of the first, second and third generation xenografts were observed. The tumor tissues, first generation and third generation of xenografts were examined by HE staining and CK5 / 6, p63, p40 immunohistochemical staining. The expression of mTOR, p-mTOR, p70S6K, pp70S6K, Akt1, p-Akt (Ser473), Erk1 / 2 and p-Erk1 / 2 were detected by Western blot in 4 cases. Results: The xenograft model was successfully established and the xenografts grew steadily and could be passaged continuously. Each xenograft histopathological type and CK5 / 6, p63, p40 expression was consistent with the patient’s tumor tissue. However, the activation of signal transduction pathway proteins in xenograft tumors from different patients was significantly different. Conclusion: The esophageal carcinoma xenografted model of human esophageal squamous cell carcinoma has been successfully established, and the model can reflect the pathological features of the patients.