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[目的]研究含新生血管靶向基序NGR的血管内皮抑素(Endostatin)多肽对人HUVEC细胞的抑制作用。[方法]固相法合成Endostatin多肽Enpep-27aa和含NGR序列多肽EnpepNGR-27aa。MTT和BrdU法检测两种多肽对HUVEC细胞增殖的影响;MTT法分析两种多肽对HUVEC细胞黏附能力的影响;Transwell法检测两种多肽对HUVEC细胞迁移的影响。[结果]当干预浓度大于10nmol/L时,两种多肽均对HUVEC细胞增殖有明显的抑制作用,且EnpepNGR-27aa组抑制率高于Enpep-27aa组(P<0.05);两种多肽均能明显降低HUVEC细胞的黏附能力,且EnpepNGR-27aa的效果更明显(P<0.05);两种多肽均显著降低HUVEC细胞的迁移能力,但两组之间无显著差异(P=0.13)。[结论]NGR基序的引入显著增强了Endostatin短肽对HUVEC细胞的增殖抑制能力,并降低了其黏附能力。
[Objective] To study the inhibitory effect of endostatin polypeptide containing neovascular targeting motif NGR on human HUVECs. [Method] Endostatin polypeptide Enpep-27aa and Npeptide N-terminal peptide EnpepNGR-27aa were synthesized by solid-phase method. MTT assay and BrdU assay were used to detect the effect of the two peptides on the proliferation of HUVECs. MTT assay was used to analyze the effect of the two peptides on HUVECs adhesion. Transwell assay was used to detect the effect of the two peptides on the migration of HUVECs. [Result] Both of the two peptides inhibited the proliferation of HUVEC significantly when the concentration was higher than 10 nmol / L, and the inhibitory rate of EnpepNGR-27 aa group was higher than that of Enpep-27 aa group (P <0.05) (P <0.05). Both peptides significantly reduced the migration ability of HUVECs, but there was no significant difference between the two groups (P = 0.13). [Conclusion] The introduction of NGR motif enhances the inhibitory effect of Endostatin short peptide on the proliferation of HUVEC cells and decreases its adhesion ability.