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本研究通过观察抑制SDF 1活性对HL 6 0细胞增殖活性的影响 ,探讨趋化因子SDF 1在维持HL 6 0细胞生存能力中的作用。培养骨髓基质细胞 ,并与HL 6 0细胞共培养 ,采用SDF 1受体CXCR4单克隆抗体阻断SDF 1生物活性后 ,用MTT法检测HL 6 0细胞活力、用流式细胞术观察HL 6 0细胞增殖周期变化、检测细胞膜表面CXCR4表达 ,同时检测CXCR4单克隆抗体应用前后HL 6 0细胞内钙离子浓度变化。结果显示 ,抗CXCR4单克隆抗体可下调HL 6 0细胞膜表面CXCR4的表达 ,同时处于G0 /G1期的细胞增多 ,处于S期的细胞减少 ,而白血病细胞存活率下调 ,细胞内钙离子浓度降低。结论 :抑制SDF 1活性可在一定程度上抑制白血病细胞的增殖
In this study, the effect of SDF-1 on the proliferation of HL-60 cells was observed to investigate the role of chemokine SDF-1 in the maintenance of HL-60 cell viability. The bone marrow stromal cells were cultured and co-cultured with HL-60 cells. The biological activity of SDF-1 was blocked by the CXCR4 monoclonal antibody of SDF-1 receptor. The viability of HL-60 cells was detected by MTT assay. The changes of cell cycle, the expression of CXCR4 on cell membrane and the change of intracellular calcium concentration in HL60 before and after the application of CXCR4 monoclonal antibody were detected. The results showed that the anti-CXCR4 monoclonal antibody down-regulated the expression of CXCR4 on the membrane surface of HL-60 cells. Meanwhile, the cells in G0 / G1 phase increased, the cells in S phase decreased, while the leukemia cell survival rate decreased and the intracellular calcium concentration decreased. Conclusion: Inhibition of SDF 1 activity can inhibit the proliferation of leukemia cells to a certain extent