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目的:通过人原代子宫内膜细胞实验研究第10号染色体缺失的磷酸酶(phosphatase and tensin homolog deleted on chromosome ten,PTEN)对子宫内膜异位症发生、发展的作用和意义。方法 :利用慢病毒载体分别在人原代子宫内膜细胞中过表达和静默PTEN基因的表达;流式细胞仪检测慢病毒感染之后不同PTEN表达组的细胞周期及凋亡变化情况。结果:PTEN过表达组人原代子宫内膜细胞G0/G1期比例增加,与对照组比较差异具有统计学意义,G2/M期比例逐渐减少,细胞周期阻滞在G0/G1期;空白对照组、PTEN过表达组、PTEN静默组的细胞凋亡率分别是(11.70±0.03)%、(15.80±0.14)%、(5.33±0.08)%。结论:PTEN可显著增加人原代子宫内膜细胞凋亡,抑制细胞周期,对子宫内膜异位症的发生发展有一定抑制作用。
Objective: To investigate the role and significance of phosphatase and tensin homolog deleted on chromosome ten (PTEN) on the occurrence and development of endometriosis in human primary endometrial cells. Methods: The lentiviral vector was used to overexpress and silence the PTEN gene expression in human primary endometrial cells respectively. The cell cycle and apoptosis of different PTEN expression groups were detected by flow cytometry. Results: The percentage of G0 / G1 phase in PTEN overexpression group was significantly increased compared with the control group, the proportion of G2 / M phase decreased gradually, and the cell cycle arrest was in G0 / G1 phase. The blank control The apoptotic rate of PTEN silencing group and PTEN overexpression group were (11.70 ± 0.03)%, (15.80 ± 0.14)% and (5.33 ± 0.08)%, respectively. Conclusion: PTEN can significantly increase the apoptosis of human primary endometrial cells, inhibit the cell cycle, and inhibit the development of endometriosis.