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目的研究吡咯喹啉醌(PQQ)对γ辐射小鼠的防护作用。方法将40只昆明小鼠随机分四组,即未照射组、单纯照射组、照射前给药组、照射后给药组。给药组小鼠口服PQQ剂量按体重计每天2mg/kg,连续给药7天。采用60Coγ射线单次全身照射,剂量5Gy。于照射后第8天,颈椎脱臼处死小鼠,收集血清、制备肝匀浆,进行各项生化指标测定。制作肝HE染色切片。结果照射后小鼠血清及肝脏中SOD、T-AOC显著下降(P<0.05),MDA显著升高(P<0.05)。照射给药组小鼠血清SOD、T-AOC含量显著升高(P<0.05),MDA含量显著减低(P<0.05);肝脏中SOD含量显著升高(P<0.05)。所有照射组小鼠肝组织都有肝板排列紊乱,出现肝细胞水肿,变性、坏死现象。结论γ辐射引发小鼠全身性的氧化应激,肝脏是重要的辐射敏感器官。PQQ对γ辐射小鼠的防护作用机制是:PQQ能直接清除自由基,同时能调动照射小鼠的全身自由基清除系统,尤其是SOD的活性,降低自由基含量。
Objective To study the protective effect of pyrroloquinoline quinone (PQQ) on γ-irradiated mice. Methods Forty Kunming mice were randomly divided into four groups: untreated group, simple irradiation group, pre-irradiation administration group and post-irradiation administration group. Oral administration of mice PQQ dose 2mg / kg body weight per day, continuous administration of 7 days. A single whole body irradiation with 60Co γ-ray dose of 5Gy. On the 8th day after irradiation, mice were sacrificed by cervical dislocation, serum was collected, and liver homogenates were prepared for biochemical determination. Making HE stained sections of liver. Results The levels of SOD and T-AOC in serum and liver were significantly decreased (P <0.05) and MDA significantly increased after irradiation (P <0.05). The levels of SOD and T-AOC in the serum of the irradiated mice were significantly increased (P <0.05), the content of MDA was significantly decreased (P <0.05), and the content of SOD in the liver was significantly increased (P <0.05). All irradiated mice liver tissue disorders have arranged in the liver plate, hepatocyte edema, degeneration, necrosis. Conclusion γ radiation induced systemic oxidative stress in mice. The liver is an important radiation-sensitive organ. The protective mechanism of PQQ to γ-irradiated mice is: PQQ can directly scavenge free radicals, and can also mobilize the systemic free radical scavenging system of irradiated mice, especially the activity of SOD, and reduce the content of free radicals.