许多生理调节所必须的因子都是很小的肽类,生物技术的发展也促进了大量小分子基因工程肽类药物的出现,因而小分子肽的分离、纯化与鉴定技术日趋重要。小分子肽的界限划分迄今未有明确定义,一般认为小分子肽的相对分子质量(Mr)均在3000以下[1]。在小分子肽的生物学功能、鉴定与 Many of the factors necessary for physiological regulation are small peptides. The development of biotechnology has also promoted the emergence of a large number of small molecule genetically engineered peptide drugs. Therefore, the separation, purification and identification of small peptide peptides are becoming more and more important. The boundaries of small peptides have not been defined so far, and the relative molecular mass (Mr) of small peptides is generally considered below 3000 [1]. The biological function of small peptides, identification and