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本研究用单克隆抗独特型抗体NP30与日本血吸虫肠相关抗原(GAA)和可溶性虫卵抗原(SEA)检测了702份不同病期及正常人群中的血清抗体,结果显示,在急性感染时,NP30抗体的检出率为98％,与GAA(94％)和SEA(98％)的无差别。在慢性感染时NP30抗体的检出率为87％,与GAA(86％)的无差别,但低于SEA(98％)的。在正常人群中,上述3种的抗体假阳性率均为3％左右,无差别。NP30的抗体滴度几何均数在急性血吸虫感染时高于GAA的,低于SEA的,在慢性感染时低于后两者,提示NP30的抗体在血吸虫感染期间出现比较早,消退较快。上述结果提示,NP30可以替代虫源性抗原,用于日本血吸虫病诊断。
In this study, the sera of 702 patients with different stages and normal population were detected with monoclonal anti-idiotypic antibody NP30, Schistosoma japonicum gut-associated antigen (GAA) and soluble egg antigen (SEA). The results showed that in acute infection, The detection rate of NP30 antibody was 98%, no difference with GAA (94%) and SEA (98%). The detection rate of NP30 antibody was 87% in chronic infection, no difference with GAA (86%), but lower than SEA (98%). In the normal population, the above three kinds of antibody false positive rate of about 3%, no difference. The geometric mean antibody titer of NP30 was higher than GAA in acute schistosomiasis infection, lower than SEA in chronic infection, and lower than the latter two in chronic infection, suggesting that NP30 antibody appeared earlier and faded faster during schistosome infection. The above results suggest that NP30 can replace insect-derived antigens for the diagnosis of schistosomiasis japonica.