论文部分内容阅读
应用组织块培养法，对大白鼠牙髓组织细胞进行了原代和传代培养，同时对培养细胞来源、生长特性进行了初步观察和鉴定。结果证实，培养的鼠牙髓细胞为来自中胚层的成纤维细胞，该细胞的细胞器发达，４～５代后的细胞生长稳定，增殖速度快，提示培养的鼠牙髓细胞适合体外实验并以选择４～５代后的细胞进行实验为宜。
The tissue culture method was used to primary and subculture the dental pulp tissue of rats. The origin and growth characteristics of cultured cells were also observed and identified. The results showed that the cultured rat dental pulp cells were mesodermal fibroblasts. The organelles were well developed. After 4-5 generations, the cells grew steadily and proliferated rapidly, which indicated that the cultured rat dental pulp cells were suitable for in vitro experiments Select 4 to 5 generations of cells after the experiment is appropriate.