以克隆表达的苏氨酸脱氨酶(L-TD)和亮氨酸脱氢酶(L-LeuDH)为催化剂,用“一锅法”将天然氨基酸转化为非天然氨基酸,最后结晶回收产物,总收率可达84%左右。所得产物经质谱和核磁共振波谱确证了结构,且HPLC检测其光学纯度>99.9%。该工艺使用的原料和酶价格低廉,又避免了中间的提取分离过程。 The natural amino acids were converted into unnatural amino acids by the “one-pot method” using the cloned L-TD and L-LeuDH as catalysts, and finally the crystal was recovered The product, the total yield of about 84%. The obtained product was confirmed by mass spectrometry and nuclear magnetic resonance spectroscopy and its optical purity was> 99.9% by HPLC. The raw materials and enzymes used in the process are cheap, and the intermediate extraction and separation process is avoided.