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目的掌握2013年克拉玛依市手足口病EV71 VP1基因特征。方法 real-time PCR方法筛选手足口病患者中EV71阳性病例,RT-PCR方法扩增VP1基因片段,结合已知亚型利用Mega软件分析测序结果。结果 59例样本中由肠道病毒引起53例,阳性率为89.8%,其中EV71 36例(67.9%)、CA16 10例(18.9%)、非EV71非CA16的其他肠道病毒7例(13.2%)。男性、女性中各病原阳性率差异无统计学意义(χ2=3.1504,P=0.207)。12株测序毒株全部为C4亚型,与C4亚型相比较基因距离为0.031~0.046,部分氨基酸序列存在变异,12例EV71至少可以划分为2个传播链,lineage 2较lineage 1序列差异较大。结论各病原对于不同性别人群侵袭力无差异,2013年克拉玛依市手足口病主要病原为EV71。克拉玛依市EV71全部是C4亚型,属于中国大陆广泛流行的基因亚型;lineage 1为2013年克拉玛依市流行的优势分支,分支内同源性较大。lineage 1将来是否继续成为克拉玛依市流行分支,需要对EV71进行连续性监测。
Objective To grasp the characteristics of EV71 VP1 gene in hand, foot and mouth disease in Karamay in 2013. Methods The EV71 positive cases were screened by real-time PCR in patients with hand-foot-mouth disease. The VP1 gene fragment was amplified by RT-PCR and sequenced with Mega software based on known subtypes. Results Among 59 samples, 53 cases were caused by enterovirus, the positive rate was 89.8%, including 36 cases of EV71 (67.9%), 10 cases of CA16 (18.9%), 7 cases of other enteroviruses (13.2% ). There was no significant difference in the positive rate of each pathogen between male and female (χ2 = 3.1504, P = 0.207). All 12 sequenced strains were all of C4 subtypes. The distance from the genotypes of C4 subtypes was 0.031-0.046, some of the amino acid sequences were variable, and 12 EV71s could be divided into at least two transmission chains. Big. Conclusion There is no difference in the invasiveness of different pathogenic bacteria among different sex groups. In 2013, the main pathogen of HFMD in Karamay was EV71. The EV71 in Karamay is all C4 subtype, belonging to the gene subtype widely prevailing in Mainland China. Lineage 1 is the popular dominant branch in Karamay in 2013, and has a high homology within the branch. Whether lineage 1 will continue to be a popular branch of Karamay in the future will require continual monitoring of EV71.