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本文研究ＮＮＫ诱发人支气管上皮细胞（ＢＥＰ２Ｄ）恶性转化及其氧化损伤机理。结果表明，５００μｇ／ｍｌ的ＮＮＫ作用后的第５代细胞具有抗血清生长能力，第１５代细胞具有锚着独立生长特性，第２５代细胞具有裸鼠体内成瘤性，提示ＮＮＫ诱发ＢＥＰ２Ｄ细胞发生了恶性转化。ＮＮＫ作用后，ＢＥＰ２Ｄ细胞产生的过氧化氢（Ｈ２Ｏ２）、超氧阴离子（Ｏ÷２）和羟自由基（·ＯＨ），以及８－羟基脱氧鸟嘌呤（ＯＨ８ｄＧ）含量均显著增高，提示ＮＮＫ致ＢＥＰ２Ｄ细胞恶性转化过程中涉及到氧化损伤的机理。
This article studies the malignant transformation of NNK-induced human bronchial epithelial cells (BEP2D) and its oxidative damage mechanism. The results showed that the 5th generation cells with 500μg / ml NNK had anti-serum growth ability, the 15th generation cells had the anchorage independent growth characteristics, the 25th generation cells had tumorigenicity in nude mice, suggesting that NNK induced BEP2D cells Malignant transformation. After NNK treatment, the content of hydrogen peroxide (H2O2), superoxide anion (O ÷ 2), hydroxyl radical (· OH) and 8-hydroxydeoxyguanine (OH8dG) in BEP2D cells were significantly increased, The mechanism of oxidative damage involved in the malignant transformation of BEP2D cells.