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为探讨抗生素相关性腹泻(AAD)的发病机制,本文研究了AAD病人、使用抗生素无腹泻病人和健康对照者的结肠碳水化合物发酵情况。材料与方法:受试对象68例,其中健康对照者17例,4周内未使用任何抗生素;使用抗生素无腹泻者44例;AAD病人7例。使用抗生素至少3天后采集粪便标本,稀释后放在充氮气的瓶中孵育0、6、24h。用气液色谱仪分析短链脂肪酸(SCFA)。测定粪便pH、左旋和右旋乳糖及糖类。所有标本进行难辨梭菌培养和毒素检测。
In order to explore the pathogenesis of antibiotic-associated diarrhea (AAD), this paper studied colon carbohydrate fermentation in patients with AAD, patients without diarrhea using antibiotics, and healthy controls. MATERIALS AND METHODS: Sixty-eight subjects were included, including 17 healthy controls, no antibiotics within 4 weeks, 44 patients without diarrhea using antibiotics and 7 patients with AAD. Stool specimens were collected at least 3 days after antibiotic use, diluted and incubated in nitrogen-filled vials for 0, 6, and 24 h. Short chain fatty acids (SCFA) were analyzed by gas liquid chromatography. Determination of stool pH, L-and dextran and carbohydrates. All specimens were tested for C. difficile culture and toxins.