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为了探究大麦种子的劣变机制,采用40℃与80%相对湿度的人工老化条件,对4个大麦品种进行老化处理,待种子风干后,测定其发芽特性、活性指标和5种抗氧化酶活性的变化。结果发现,大麦发芽特性指标在老化过程中呈反S型下降趋势;大麦种子浸出液相对电导率变化趋势不稳定,与发芽特性指标相关性不显著,四氮唑还原强度先上升后下降,过氧化氢酶活性先上升后下降,过氧化物酶、超氧化物歧化酶、谷胱甘肽还原酶、脱氢抗坏血酸还原酶活性总体呈下降趋势。种子发芽速率相关指标适合对大麦贮藏性的评价;种子浸出液相对电导率不能准确、灵敏地反映大麦种子的活性状况;四氮唑还原强度可作为部分大麦品种的活性评定指标;过氧化氢酶、过氧化物酶、谷胱甘肽还原酶活性与大麦种子老化过程密切相关。
In order to explore the mechanism of deterioration of barley seeds, four barley varieties were aged under artificial aging conditions of 40 ℃ and 80% relative humidity. After the seeds were air-dried, the germination characteristics, activity indexes and five antioxidant enzyme activities The change. The results showed that the germination characteristics of barley showed an inverse S-shaped trend during the aging process; the relative conductivity of barley seed leachate showed a trend of instability with no significant correlation with germination characteristics; the reduction strength of tetrazolium first increased and then decreased; The activity of hydrogenase firstly increased and then decreased, and the activity of peroxidase, superoxide dismutase, glutathione reductase and dehydroascorbate reductase tended to decrease. The relative indexes of seed germination rate are suitable for evaluating the storage of barley; the relative conductivity of seed leachate can not accurately and sensitively reflect the activity status of barley seed; the reduction strength of tetrazolium can be used as the activity evaluation index of some barley varieties; the catalase, Peroxidase and glutathione reductase activity are closely related to the aging process of barley seeds.