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测定多聚核糖体含量及多聚程度的分布在研究生物组织的生理生化现象和代谢过程中有重要意义。其定量分离的报道很多。本文利用新型离心转头、通过变更密度梯度配比,离心时间和离心速度3个参量,经过多次实验,获得一个新的制备方法,兹将结果介绍如下。先采用Dupont/Soruall TH641水平转头,离心管加入40%～10%线性蔗糖梯度,再加入粗制鼠肝多聚核糖体,温度10℃,转速3500r/min,离90min。再采用日立scp-85H超速离心机的RPV-50T垂直转头,40PA离心管,管底加20mol/L ST KM,其上是55%～15%
It is important to determine the distribution of polysaccharide content and degree of aggregation in the study of physiological and biochemical phenomena and metabolic process of biological tissues. There are many reports of its quantitative separation. In this paper, using a new type of centrifugal rotor, by changing the density gradient ratio, centrifugation time and centrifugal velocity of three parameters, after a number of experiments to obtain a new preparation method, the results are as follows. The first use of Dupont / Soruall TH641 level rotor, centrifuge tube was added 40% to 10% linear sucrose gradient, then add the crude rat liver polysome ribosome, temperature 10 ° C, speed 3500r / min, away from 90min. Then use Hitachi scp-85H ultracentrifuge RPV-50T vertical rotor, 40PA centrifuge tube, the bottom of the tube plus 20mol / L ST KM, which is 55% to 15%