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目的:探讨UV-B辐射胁迫下黄连的抗逆生理机制,为黄连栽培及提高黄连品质提供参考依据。方法:通过设置UV-B辐射时间及不同强度梯度,运用聚丙烯酰胺垂直板电泳技术,对各处理组的黄连同工酶及相关逆境指标进行了分析。结果:UV-B辐射3 h后,SOD1(Rf=0.125)、SOD2(Rf=0.312)、CAT1(Rf=0.428)、POD3(Rf=0.290)、POD4(Rf=0.636)谱带被先后诱导,随着UV-B辐射时间的增长,这些谱带开始丢失或减弱,尤其处于重度UV-B胁迫下,CAT1(Rf=0.428)、POD3(Rf=0.290)谱带会提前丢失;此外,UV-B辐射使得叶片中MDA、可溶性糖、脯氨酸含量显著高于对照组,但UV-B辐射7 h后,除UL组脯氨酸上升外,各组MDA、可溶性糖、脯氨酸含量开始有所下降,可溶性蛋白谱带数增多。结论:短时间的UV-B辐射会诱导黄连增加抗氧化酶同工酶的表达量及谱带数,积累可溶性糖、可溶性蛋白等抗性物质来提高自身抗性能力,UV-B辐射持续7 h后,黄连自身调节系统被破坏,代谢开始紊乱,同工酶谱带开始丢失及减弱,可溶性蛋白条带增多变宽。
OBJECTIVE: To investigate the physiological mechanism of Coptis chinensis Franch under UV-B radiation stress and provide references for the cultivation of Coptis chinensis and improve the quality of Coptis chinensis. Methods: The UV-B radiation time and different intensity gradients were set up. Polyacrylamide vertical plate electrophoresis technique was used to analyze the isozymes of Coptis chinensis and their relative stress indexes. Results: The bands of SOD1 (Rf = 0.125), SOD2 (Rf = 0.312), CAT1 (Rf = 0.428), POD3 (Rf = 0.290) and POD4 (Rf = 0.636) With the increase of UV-B radiation time, these bands began to lose or weaken. Especially under the severe UV-B stress, the bands of CAT1 (Rf = 0.428) and POD3 (Rf = 0.290) B radiation, the contents of MDA, soluble sugar and proline in the leaves were significantly higher than those in the control group. However, after prolonged UV-B irradiation for 7 h, the contents of MDA, soluble sugar and proline increased Decreased, the number of soluble protein bands increased. Conclusion: Short-term UV-B radiation induces the increase of the expression of antioxidant enzymes and the number of bands in the Coptis chinensis, and accumulates soluble sugar and soluble protein to enhance the self-resistance. UV-B radiation lasts for 7 h, Coptis its own regulatory system is destroyed, the metabolism began to disorder, isozyme bands began to lose and weakened, soluble protein bands increased broadening.