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硫氧化还原蛋白(thioredoxin,TRX)1是一种普遍存在的12kD的蛋白质,可产生抗氧化和抗炎作用。由巨噬细胞产生的截断形式称为TRX80,其可诱导促炎细胞因子的上调。TRX80还促进小鼠腹膜和人巨噬细胞向促炎M1表型的分化。方法:使用特异性酶联免疫吸附试验测定血浆TRX1和TRX80水平。分别使用SensoLyte~ 520去整合素-金属蛋白酶(a disintegrin and metalloprotease domain,ADAM)10
Thioredoxin (TRX) 1 is a ubiquitous 12 kD protein that produces anti-oxidant and anti-inflammatory effects. The truncated form produced by macrophages is called TRX80, which induces the upregulation of proinflammatory cytokines. TRX80 also promotes the differentiation of mouse peritoneal and human macrophages to the pro-inflammatory M1 phenotype. Methods: Plasma TRX1 and TRX80 levels were measured using a specific enzyme-linked immunosorbent assay. SensoLyte ~ 520 were used respectively to disintegrin and metalloprotease domain (ADAM) 10