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肺癌是最常见的恶性肿瘤之一,其发病率逐年升高,而且死亡率很高.肺癌患者约有50%可以手术治疗,另外50%患者由于转移等原因不能手术而需化疗等治疗,此外经过手术治疗的患者也常需化疗,然而许多患者常化疗失败(无效或效果不佳),非小细胞肺癌(NSCLC)对化疗尤其不敏感.研究已经表明,肿瘤化疗失败的重要原因之一是肿瘤产生耐药性,而耐药性的产生大部分由于多重耐药基因(MDR1)及其产物P-糖蛋白(Pgp)的表达增高,因此许多专家建议在化疗之前进行MDR1检查,以确定治疗方案,因此克服肿瘤耐药性是肿瘤治疗中急需解决的问题.反义技术是一种抑制基因表达的新技术,在抗病毒和抗肿瘤方面的研究结果令人鼓舞,显示了其在基因治疗方面的广阔应用前景.我们在体外用阿霉素诱导了一株肺腺癌细胞系的耐药株(GAOK),其MDR1基因表达增高.为了逆转GAOK细胞的耐药性,通过逆转录病毒介导的基因转移,将MDR1反义RNA导入耐药GAOK细胞
Lung cancer is one of the most common malignancies. Its incidence increases year by year, and the mortality rate is very high. About 50% of lung cancer patients can be treated with surgery. The other 50% of patients can not be treated due to metastasis and need chemotherapy and other treatments. Surgically treated patients often need chemotherapy, but many patients often fail chemotherapy (ineffective or ineffective), and non-small cell lung cancer (NSCLC) is particularly insensitive to chemotherapy. Studies have shown that one of the important reasons for the failure of cancer chemotherapy is Tumors develop drug resistance, and drug resistance is largely due to the increased expression of multiple drug resistance genes (MDR1) and their product P-glycoprotein (Pgp). Therefore, many experts recommend that MDR1 tests be performed before chemotherapy to determine the treatment. The solution, therefore overcoming the resistance of tumors is an urgent problem to be solved in the treatment of cancer. Antisense technology is a new technology that inhibits gene expression. The results of anti-virus and anti-tumor research are encouraging and show that it is in gene therapy. The wide application prospects. We used a doxorubicin to induce a drug resistant strain (GAOK) of lung adenocarcinoma cell line in vitro. Its MDR1 gene expression was increased. In order to reverse the resistance of GAOK cells, through the inverse Retrovirus-mediated gene transfer, the MDR1 antisense RNA into cells resistant GAOK