论文部分内容阅读
本实验应用豚鼠抗胰岛素抗体及胶体金探针对常规Epon812包坦后的大鼠胰腺组织胰岛素分泌细胞进行电镜免疫金染色,获得较满意的结果。材料与方法:取大鼠新鲜胰腺组织,常规透射电镜制样。半薄切片光镜下精确定位胰岛。超薄切片捞于不锈钢载网上。免疫金染色程序为:①8％高碘酸钠室温腐蚀20分钟;②0.01MPBS(含1％BSA)pH7.2洗涤3次,每次5分钟;③5％BSA孵育20分钟,以封闭非特异性结合位点;④豚鼠抗胰岛素抗体
In this study, guinea pig anti-insulin antibody and gold colloidal gold probe on conventional Epon812 package of pancreatic tissue insulin secreting cells after gold staining by electron microscopy to obtain more satisfactory results. MATERIALS AND METHODS: Fresh rat pancreatic tissue was taken for routine transmission electron microscopy. Semi-thin slice light microscopy accurate positioning islet. Slim slice fishing in stainless steel net. Immunogold staining procedures: ① 8% sodium periodate at room temperature for 20 minutes; ② 0.01MPBS (containing 1% BSA) pH7.2 washed three times for 5 minutes each; ③ 5% BSA incubated for 20 minutes to block non-specific binding Site; ④ guinea pig anti-insulin antibody