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本实验研究了转输615系小鼠脾LAK细胞和LAK—88/DC细胞系对615小鼠白血病(L615)的免疫防治效应,以及继承性化学免疫治疗作用。实验结果表明,攻击前二十一天转输LAK细胞或LAK—88/DC细胞系,并每日腹腔注射培养上清液一周,可以诱导正常受鼠产生对L615明显的免疫预防效应,分别使实验组80%的小鼠长期存活;单独注射培养上清液也有效,可使60%的小鼠长期存活。在攻击后五天,用环磷酰胺(CY:180mg/kg)进行化疗并转输LAK细胞或LAK—88/DC细胞系,则能延长晚期L615病鼠的平均存活时间,并可使实验组25%～28.6%的小鼠长期存活。
In this study, we investigated the immunocontrol efficacy of 615-derived leukemia (615) leukemia (LA615) transfected with 615-derived mouse LAK cells and LAK-88 / DC cell lines and the effect of inherited chemotherapeutic immunotherapy. The results showed that LAK cells or LAK-88 / DC cell lines were transfused 21 days prior to challenge and daily intraperitoneal injections of the supernatant for one week induced a significant immunoprotective effect on L615 in normal mice Eighty percent of the mice in the experimental group survived long-term; single injection of the supernatant was also effective, allowing 60% of the mice to survive long-term. Five days after challenge, chemotherapy with cyclophosphamide (CY: 180 mg / kg) and transfection into either LAK cells or LAK-88 / DC cell lines prolongs the mean survival of late-stage L615-challenged mice and allows the experimental group Twenty-five percent to 28.6% of mice survive long-term.