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目的分析风疹IgG抗体定量检测的主要影响因素及影响趋势。方法在拥有相对固定的检测环境和条件下,使用稳定性能好的德国维润赛润风疹定量检测试剂盒,用酶联免疫法检测IgG抗体浓度。结果基层单位样品少,手工操作多,在环境温度、孵育温度、洗板严格控制下,样品加样时间对定量值的影响很大。结论影响定量检测准确性的因素很多,为保证结果重复性所用的仪器、加样器、温度计等要经过检定,控制环境温度,基层手工操作控制好加样时间,根据样品量的数量,增加阳性对照,是保证结果定量准确的有效手段。
Objective To analyze the main influential factors and influence trend of quantitative detection of rubella IgG antibody. Methods In the presence of a relatively fixed detection environment and conditions, the use of a good stability of the German Wei Run Race Run rubella quantitative detection kit, using enzyme-linked immunosorbent assay IgG antibody concentration. Results There were a few sample units in the grassroots units and many manual operations. Under the control of ambient temperature, incubation temperature and washing plate, sample loading time had a great influence on the quantitative value. Conclusion There are many factors affecting the accuracy of quantitative detection. The instruments used for ensuring the repeatability of results, such as sample changers and thermometers, should be tested to control the ambient temperature and the time required for sample preparation by manual control. According to the quantity of samples, the positive Control, is to ensure quantitative and accurate results of the effective means.