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作者利用大鼠在CS_2长期作用下进行实验研究,以阐明CS_2对心血管系统过早衰老的危险性以及选择作用的程度。选用体重为240±20 g的雄性大鼠,在CS_2浓度为112±8、13±4、1.2±0.3mg/m~3下吸入4个月,每组25～30只大鼠、另设一组对照组。在4个月实验结束后,以及实验结束9个月后分别对其机能、生化、形态等指标进行综合性评价。指标采用:动脉压、心率及胆固醇、磷脂、β-脂蛋白、主动脉中的羟脯氨酸、氨基己糖、己糖醛酸的含量、测定心肌中肌原纤维与结缔组织的比值、主动脉与肌肉弹性型血管层厚度比值、心脏外界大小与左右心室壁厚度比值、组织化学(当pH 4.0时甲苯胺蓝染色)、测定动脉内膜下层的酸性粘多糖类含量;慢性实验期间还观察动物一段状况、体重、血尿等指标。
The authors used rat CS2 long-term experimental study to clarify the CS2 risk of premature aging of the cardiovascular system and the extent of the role of choice. Male rats weighing 240 ± 20 g were selected and inhaled for 4 months at concentrations of 112 ± 8, 13 ± 4 and 1.2 ± 0.3 mg / m 3 of CS 2, with 25 to 30 rats in each group. Group control group. After 4 months of experiment, and 9 months after the end of the experiment, their functions, biochemistry, morphology and other indicators were evaluated comprehensively. The indexes included: arterial pressure, heart rate and cholesterol, phospholipid, β-lipoprotein, hydroxyproline, hexosamine and hexuronic acid in the aorta, and the ratio of myofibrillar to connective tissue in myocardium Arterial and muscular elastic blood vessel layer thickness ratio, heart size and left ventricular wall thickness ratio, histochemistry (when pH 4.0 when toluidine blue staining), determination of arterial intima sublingual mucopolysaccharide content; chronic experimental period also Observation of animal status, weight, hematuria and other indicators.