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目的:观察梓醇与小檗碱及其配伍对胰岛素抵抗3T3-L1脂肪细胞葡萄糖消耗及这一过程中葡萄糖转运子-4(Glut-4)、胰岛素受体底物-1(IRS-1)和胰岛素受体底物-1丝氨酸307(IRS-1 Ser307)磷酸化蛋白表达的影响。方法:采用高糖联合高胰岛素诱导3T3-L1脂肪细胞产生胰岛素抵抗,分别给予小檗碱、梓醇、小檗碱+梓醇、盐酸罗格列酮进行干预,以葡萄糖氧化酶法检测培养液中葡萄糖消耗量,以Western Blot法检测蛋白的表达。结果:与模型组相比,小檗碱能增加培养液中葡萄糖的消耗(P<0.01),但对Glut-4蛋白的表达无影响;梓醇、小檗碱+梓醇均能显著增加培养液中葡萄糖的消耗(P<0.01),并使细胞中Glut-4蛋白的表达增强(P<0.05),且小檗碱+梓醇组的效应优于梓醇组及小檗碱组;与模型组相比,小檗碱与梓醇及其配伍对IRS-1的表达没有显著性影响,但能降低IRS-1 Ser307磷酸化蛋白表达。结论:小檗碱、梓醇及其配伍能改善胰岛素抵抗3T3-L1脂肪细胞的胰岛素敏感性,其作用机制与罗格列酮不同。
OBJECTIVE: To observe the glucose consumption of insulin-resistant 3T3-L1 adipocytes by sterols and berberine and their combination, and glucose transporter-4 (Glut-4) and insulin receptor substrate-1 (IRS-1) in this process. And the effect of insulin receptor substrate-1 serine 307 (IRS-1 Ser307) phosphorylation protein expression. METHODS: Insulin resistance was induced by 3T3-L1 adipocytes induced by high glucose combined with high insulin. Berberine, sterol, berberine + sterol, rosiglitazone hydrochloride were used to intervene, and glucose oxidase assay was used to detect the broth. Glucose consumption was measured by Western Blot assay for protein expression. Results: Compared with the model group, berberine can increase glucose consumption in the culture fluid (P<0.01), but has no effect on the expression of Glut-4 protein; sterol, berberine + sterol can significantly increase the culture Glucose consumption in the fluid (P<0.01) increased the expression of Glut-4 protein in the cells (P<0.05), and the effect of berberine + sterol group was better than that of sterol group and berberine group; Compared with the model group, berberine and sterols had no significant effect on the expression of IRS-1, but it could reduce the expression of IRS-1 Ser307 phosphorylation protein. CONCLUSION: Berberine, sterols and their compatibility can improve the insulin resistance of insulin-resistant 3T3-L1 adipocytes, and its mechanism of action is different from that of rosiglitazone.