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本试验利用北京正负电子对撞机直线加速器E2束流打靶产生高能次级混合粒子场,模拟次级宇宙射线,分别以0、109、145、195、284和560Gy剂量处理两个冬小麦品种ZY9和ZH7,并与相同剂量的60Coγ射线相比较,研究混合粒子场辐射处理对小麦幼苗生长和微卫星(SSR)标记谱带变化的影响。试验结果表明,随着混合粒子场处理剂量的增加,小麦幼苗生长受到的损伤逐渐增加。SSR标记分析结果发现,小麦B基因组的多态性位点频率占总多态性频率的46%,在3个基因组中最高,可能为“热点”突变基因组。高能混合粒子场对冬小麦幼苗的生长抑制和微卫星DNA的损伤效应大于γ射线。
In this experiment, a high-energy secondary mixed particle field was generated by beam-to-beam direct current electron collider E2 in Beijing to simulate secondary cosmic rays. Two winter wheat varieties, ZY9, were treated with 0,109,145,195,284 and 560Gy doses respectively. And ZH7, and compared with the same dose of 60Coγ ray to study the effect of mixed particle field radiation on the growth of wheat seedling and the change of microsatellite (SSR) bands. The results showed that with the increase of dose of mixed particle field, the damage of wheat seedling growth was gradually increased. The result of SSR analysis showed that the polymorphism loci of wheat B genotype accounted for 46% of the total polymorphism frequency, which was the highest among the three genomes and might be the “hot spot” mutant genome. The effect of high energy mixed particle field on the growth inhibition and microsatellite DNA damage of winter wheat seedlings was greater than that of γ-rays.