论文部分内容阅读
目的:观察气滞血瘀证模型大鼠体内硫化氢/胱硫醚-γ-裂解酶(H2S/CSE)、硫化氢/胱硫醚-β-合成酶(H2S/CBS)变化。方法:将Wistar大鼠随机分成4组:对照组、模型组、血府逐瘀汤低剂量组(34.5g/kg)、血府逐瘀汤组高剂量组(103g/kg)。采用多因素联合刺激法建立气滞血瘀模型。运用分光光度法测定血浆、肝组织中H2S含量,利用RT-PCR法测定肝组织中CSE、CBS基因表达。采用免疫组化法检测颈总动脉平滑肌细胞中CSE表达情况。结果:模型组血浆H2S含量(42.64±1.67)μmol/L显著低于对照组(105.52±3.07)μmol/L(P<0.01),血府逐瘀汤低剂量(34.5g/kg)血浆H2S含量(79.34±0.57)μmol/L显著高于模型组(42.64±1.67)μmol/L(P<0.01);模型组肝组织中H2S含量(44.54±2.33)μmol/L显著低于对照组(70.35±2.47)μmol/L(P<0.01),血府逐瘀汤组低剂量组肝组织中H2S含量(63.99±4.53)μmol/L显著高于模型组(44.54±2.33)μmol/L(P<0.01);模型组肝组织CSE表达水平显著低于对照组(P<0.01),血府逐瘀汤组低剂量组肝组织CSE表达水平显著高于模型组(P<0.05),肝组织中的CBS表达变化没有统计学意义(P>0.05)。模型组CSE蛋白表达量显著低于对照组(P<0.01),血府逐瘀汤组低剂量组CSE蛋白表达量显著高于模型组(P<0.05)。结论:采用多因素联合刺激法建立的气滞血瘀证大鼠中H2S/CSE体系下调。
Objective: To observe the changes of hydrogen sulfide / cystathionine-γ-lyase (H2S / CSE) and hydrogen sulfide / cystathionine-β-synthase (H2S / CBS) in rats with qi stagnation and blood stasis syndrome. Methods: Wistar rats were randomly divided into 4 groups: control group, model group, Xuefu Zhuyu Decoction low dose group (34.5g / kg) and Xuefu Zhuyu Decoction high dose group (103g / kg). Gas-stagnation and blood stasis model was established by multi-factor combined stimulation. The content of H2S in plasma and liver tissue was measured by spectrophotometry, and the expression of CSE and CBS in liver tissue was determined by RT-PCR. The expression of CSE in common carotid artery smooth muscle cells was detected by immunohistochemistry. Results: The content of H2S in model group (42.64 ± 1.67) μmol / L was significantly lower than that in control group (105.52 ± 3.07) μmol / L (P <0.01), and that of Xuefuzhuyu Decoction low dose (79.34 ± 0.57) μmol / L was significantly higher than that of model group (42.64 ± 1.67) μmol / L (P <0.01). The content of H2S in liver tissue in model group was significantly lower than that in control group (44.54 ± 2.33) μmol / L 2.47) μmol / L (P <0.01). The content of H2S in the liver tissue of Xuefuzhuyu Decoction group (63.99 ± 4.53μmol / L) was significantly higher than that of model group (44.54 ± 2.33μmol / L) ). The expression level of CSE in the liver tissue of the model group was significantly lower than that of the control group (P <0.01). The CSE expression level in the liver tissue of the low-dose Xuefuzhuyu Decoction group was significantly higher than that in the model group (P <0.05) There was no significant difference in expression (P> 0.05). The expression of CSE protein in model group was significantly lower than that in control group (P <0.01). The expression of CSE protein in low-dose Xuefuzhuyu Decoction group was significantly higher than that in model group (P <0.05). Conclusion: H2S / CSE system down-regulated in qi stagnation and blood stasis syndrome rats established by multi-factor combined stimulation.