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目的:探讨长链非编码核糖核酸AK093987(lnc RNA AK093987)在结肠癌组织中的表达及其临床意义。方法:收集2011年1月至2015年12月四川内江市第一人民医院收治的65例结肠癌患者的肿瘤组织及癌旁组织和15例先天性巨结肠和各种原因结肠破裂穿孔、肠扭转、嵌顿疝患者的正常结肠组织。通过Real-time PCR法检测lnc RNA AK093987在65例结肠癌组织、癌旁正常组织及结肠癌细胞中的表达,CCK8法检测转染si RNAAK093987下调lnc RNA AK093987表达的结肠癌Lo Vo细胞的增殖。采用Chi-Square检验和Kaplan-Meier法分别分析lnc RNA AK093987表达与临床病理参数、生存时间和预后的关系。Cox回归分析影响结肠癌患者DFS和OS的因素。结果:lnc RNA AK093987在结肠癌组织中的表达明显高于癌旁组织和正常结肠组织(7.125±1.398 vs 1.058±0.070、1.092±0.049,均P<0.01)。下调lnc RNA AK093987表达的结肠癌Lo Vo细胞增殖显著降低(P<0.05)。lnc RNA AK093987的表达与疾病分期、淋巴结转移、远处转移、肿瘤分化、血清CEA水平和生存状态明显相关(均P<0.05);而与患者的性别、年龄及肿瘤部位无显著相关(均P>0.05);高表达lnc RNA AK093987患者的中位DFS和OS均较低表达者明显缩短[DFS:(13.00±1.49)vs(27.01±1.87)个月;OS:(27.00±3.32)vs(43.72±3.08)个月,均P<0.01];lnc RNA AK093987的表达、远处转移及临床分期是结肠癌独立的预后因素(P<0.05)。此外,下调lnc RNA AK093987的表达能够明显抑制肿瘤细胞的增殖。结论:lnc RNA AK093987参与结肠癌的发生发展,lnc RNA AK093987的表达与疾病分期、淋巴结转移、远处转移、肿瘤分化、血清CEA水平和患者生存状态有关,可作为潜在的结肠癌诊断和预后评估的分子标志物。
Objective: To investigate the expression of long-chain non-coding RNA AK093987 (lnc RNA AK093987) in colon cancer and its clinical significance. Methods: From January 2011 to December 2015, the first People’s Hospital of Neijiang City, Sichuan Province, 65 cases of colon cancer patients with tumor tissue and adjacent tissues and 15 cases of Hirschsprung’s disease and a variety of reasons colon rupture perforation, intestinal torsion , Incarcerated hernia patients with normal colon tissue. Real-time PCR was used to detect the expression of lncRNA AK093987 in 65 colon cancer tissues, adjacent normal tissues and colon cancer cells. CCK8 assay was used to detect the proliferation of colon cancer LoVo cells transfected with siRNA-AK093987. Chi-Square test and Kaplan-Meier method were used to analyze the relationship between lncRNA AK093987 expression and clinicopathological parameters, survival time and prognosis. Cox regression analysis of factors affecting colon and cancer DFS and OS. Results: The expression of lncRNA AK093987 in colon cancer tissues was significantly higher than that in paracancer tissues and normal colon tissues (7.125 ± 1.398 vs 1.058 ± 0.070, 1.092 ± 0.049, all P <0.01). Proliferation of LoVo cells was significantly decreased (P <0.05). The expression of lnc RNA AK093987 was significantly correlated with the stage of disease, lymph node metastasis, distant metastasis, tumor differentiation, serum CEA level and survival status (all P <0.05), but not with the gender, age and tumor location > 0.05). Patients with high expression of lncRNA AK093987 had significantly lower median DFS and OS than those with low expression (DFS: (13.00 ± 1.49) vs (27.01 ± 1.87) months vs OS: (27.00 ± 3.32 vs ± 3.08) months, all P <0.01]. The expression of lncRNA AK093987, distant metastasis and clinical stage were independent prognostic factors of colon cancer (P <0.05). In addition, downregulation of lnc RNA AK093987 expression can significantly inhibit tumor cell proliferation. Conclusion: lncRNA AK093987 is involved in the development of colon cancer. The expression of lncRNA AK093987 is correlated with the stage of the disease, lymph node metastasis, distant metastasis, tumor differentiation, serum CEA level and the survival status of patients, which may be used as a potential diagnostic and prognostic factor for colon cancer Molecular markers.