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目的:探讨刺参糖胺聚糖的抗仙台病毒(Sendai virus,SV)作用及其作用机制。方法:选用不同浓度刺参糖胺聚糖作用于仙台病毒感染BHK21细胞的多个环节,倒置显微镜观察病毒致细胞病变效应,MTT法检测细胞活性;同时以SV滴鼻感染小鼠,观察刺参糖胺聚糖对感染病毒小鼠血凝抑制抗体产生的影响。结果:刺参糖胺聚糖浓度大于3.2mg/ml时表现出细胞毒性作用。浓度在0.25～0.2mg/ml范围时,刺参糖胺聚糖作用病毒吸附组和病毒复制组能明显抑制细胞病变,MTT结果也表明该两组显示较好的细胞活性,且具有一定的量效关系,无细胞毒性作用;但SJ-GAG直接作用病毒组、预处理细胞组以及抑制病毒穿入组无明显抗病毒作用。动物实验表明刺参糖胺聚糖可促进小鼠血凝抑制抗体的产生。结论:刺参糖胺聚糖的抗仙台病毒作用主要通过抑制病毒对靶细胞的吸附以及抑制病毒复制而实现,并能促进小鼠对病毒感染的免疫应答。
Objective: To investigate the anti-Sendai virus (SV) effect and its mechanism of action of glycosaminoglycans from sea cucumber. METHODS: Different concentrations of glycosaminoglycans were used to treat several aspects of Sendai virus infection in BHK21 cells. The cytopathogenic effect of virus was observed by inverted microscope. Cell viability was measured by MTT assay. At the same time, mice were infected with SV intranasally and observed. Effect of glycosaminoglycans on the production of hemagglutination inhibitory antibodies in infected mice. RESULTS: When the concentration of glycosaminoglycan in sea cucumber was greater than 3.2 mg/ml, it showed cytotoxicity. When the concentration was in the range of 0.25-0.2 mg/ml, the virus adsorption group and virus replication group of sea cucumber could obviously inhibit the cytopathic effect. The MTT results also showed that the two groups showed good cell activity and had a certain amount of The effect relationship was not cytotoxic; however, the SJ-GAG direct effect virus group, the pretreatment cell group, and the inhibitory virus penetration group had no significant antiviral effect. Animal experiments show that sea cucumber glycosaminoglycan can promote the production of hemagglutination inhibition antibodies in mice. CONCLUSION: The anti-Sendai virus effect of glycosaminoglycans is mainly achieved by inhibiting the adsorption of virus to target cells and inhibiting virus replication, and can promote immune responses to virus infection in mice.