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目的:观察电针治疗对分级坐骨神经压迫模型小鼠的镇痛效应和脊髓炎性细胞因子表达的影响。方法:将雄性C57/BL6小鼠随机分为假手术组(N0)、结扎一道组(N1)与一道电针组(N1+E)、结扎两道组(N2)与两道电针组(N2+E)、结扎四道组(N4)与四道电针组(N4+E)。N0组只游离神经,不作任何结扎;其余各组用分级坐骨神经结扎法建立动物模型,其中N1+E、N2+E和N4+E组分别取双侧环跳、足三里穴进行电针治疗,均隔日1次,共7次。在术前和术后第2、4、6、8、10、12、14天测定小鼠右后爪机械痛阈值;术后第14天取腰段脊髓,用酶联免疫吸附法(ELISA)测定脊髓白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的浓度。结果:与N0组比较,各手术组小鼠的机械痛阈值在造模后随时间变化而呈下降趋势,其中N1、N1+E组在术后第6天起明显降低(P<0.05),N2、N2+E、N4、N4+E组在术后第4天起显著降低(P<0.05)。与N1组比较,电针可明显提高N1+E组的术后机械痛阈值(P<0.05);但与N2和N4组比较,电针对N2+E和N4+E组的机械痛改变不明显(P>0.05)。与N0组比,N1、N2、N4组脊髓IL-1β、IL-6、TNF-α表达均显著上调(P<0.05);与N1组比较,N1+E组的IL-1β、IL-6、TNF-α表达均显著降低(P<0.05);与N2组比较,N2+E组仅IL-1β的表达下调(P<0.05);与N4组比较,N4+E组各炎性因子均无明显改变(P>0.05)。结论:电针可部分减轻小鼠分级坐骨神经缩窄模型引起的痛觉过敏,并不同程度降低脊髓IL-1β、IL-6和TNF-α的表达。
Objective: To observe the effect of electroacupuncture on the analgesic effect and the expression of inflammatory cytokines in sciatic nerve compression rats. Methods: Male C57 / BL6 mice were randomly divided into sham operation group (N0), ligation of one group (N1) and one electroacupuncture group (N1 + E), ligation of two groups (N2) and two electroacupuncture groups N2 + E), ligation of four groups (N4) and four electro-acupuncture group (N4 + E). N0 group was only free of nerve, without any ligation; the rest of the groups were established by animal model of sciatic nerve ligation, in which N1 + E, N2 + E and N4 + E group were taken bilateral ring jump, Zusanli electroacupuncture treatment, Every other day, a total of 7 times. The mechanical threshold of right hind paw in mice was measured preoperatively and on the 2nd, 4th, 6th, 8th, 10th, 12th, 12th and 14th day after operation. The spinal cord of the lumbar spinal cord was taken on the 14th postoperative day, The concentrations of IL-1β, IL-6 and TNF-α in the spinal cord were measured. Results: Compared with N0 group, the mechanical pain threshold of each operation group decreased with the time after modeling. The N1, N1 + E group decreased significantly on the 6th day after operation (P <0.05) The N2, N2 + E, N4 and N4 + E groups decreased significantly on the 4th postoperative day (P <0.05). Compared with N1 group, electroacupuncture could significantly increase the postoperative mechanical pain threshold in N1 + E group (P <0.05), but there was no significant change in mechanical pain in N2 + E and N4 + E groups compared with N2 and N4 groups (P> 0.05). Compared with N0 group, the expressions of IL-1β, IL-6 and TNF-α in N1, N2 and N4 groups were significantly increased (P <0.05) (P <0.05). Compared with N2 group, the expression of IL-1β was only down-regulated in N2 + E group (P <0.05). Compared with N4 group, all the inflammatory factors in N4 + E group No significant change (P> 0.05). Conclusion: Electroacupuncture can reduce the hyperalgesia induced by sciatic nerve narrowing model in mice and reduce the levels of IL-1β, IL-6 and TNF-α in spinal cord to some extent.