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为了了解海带受精时间和孢子囊形成的关系,以B013品系为材料,在2014-2015年度,先后在5月、6月、7月和8月,四次完成受精,培育了B013幼苗,并在大连旅顺海带栽培区开展了栽培试验。结果表明,5、6月份培育的藻体,分别有100%和80%的藻体在1月中旬开始在顶端1/3处形成首批宽度6 cm左右,长度50~60 cm左右的孢子囊群,这些孢子囊在随后的3个月中,逐渐释放了孢子,并随着藻体的不断生长,孢子囊形成区域的藻体逐渐褪去,到了9月中、下旬,藻体全部区域形成第二批孢子囊群;7、8月份培育的藻体没有在冬季形成首批孢子囊,藻体持续生长,只在9月中、下旬,在藻体的全部,形成了唯一的一次孢子囊群。所有从首批、次批孢子囊释放出来的游孢子经过附着、后期培育测试,都能够正常的形成健康的幼孢子体。这些结果表明,B013孢子囊形成规律不同于双亲中的栽培种群父本,而更加像野生的母本,总是在藻体生长到一定程度时候,在顶端先少量形成孢子囊群,之后在秋天日照时间变短,海水温度开始下降的时候大批量形成孢子囊群,藻体随后流失。该研究结果为后续研究B013孢子囊的形成机制提供了一定的理论依据。
In order to understand the relationship between the timing of fertilization and the formation of sporangium, B013 strain was used as material to fertilize B013 four times in May, June, July and August in 2014-2015, Dalian Lushun kelp cultivated areas to carry out cultivation experiments. The results showed that 100% and 80% of the algae cultured in May and June respectively formed the first batch of sporangia about 6 cm in width and 50 ~ 60 cm in length from the middle 1/3 of January In the subsequent 3 months, these sporangia gradually released spores, and with the continuous growth of the algae, the algae in the sporulation zone gradually faded away. In the middle and late September, the whole area of the algae formed Two groups of sporocysts; in July and August, the algae formed did not form the first batch of sporangium in winter, and the algal bodies continued to grow. Only in the middle and late September, all the algae formed a single sporangium . All the spores released from the first batch and the second batch of sporangiums were able to form healthy sporozoites normally after attachment and subsequent incubation tests. These results indicate that the formation of the B013 sporocysts is different from the male parent of the cultivated population in the parent and more like the wild female and always forms a small amount of sporocysts at the top when the growth of the algae reaches a certain level. Shorter sunshine time, seawater temperature began to decline when the mass sporozoite formation, algae then lost. The results provide a theoretical basis for the subsequent study on the formation mechanism of B013 sporangium.