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【目的】查明养殖刺参附着期“化板症”的病原及其来源,并获得该病的治疗药物。【方法】分别对化板症状较为典型的3家育苗场的发病幼体进行病原学分析,对可疑病原进行人工回接感染并进行形态学、生理生化和16SrDNA序列分析鉴定,对各养殖场育苗系统,包括水源、养殖池水、池底污物、附着基板和饵料进行细菌学分析,对病原菌进行药敏测试。【结果】从所有病参中分离得到1种优势菌株,人工回接感染证明它对健康刺参有较强的致病性,且感染病参与自然发病刺参的症状相同。鉴定出“化板症”的病原为弧菌Vibriosp.。养殖系统中除水源的细菌浓度没有超标污染(<50cfu/mL)外,其余均检出大量细菌(>1×105cfu/mL);病原来源较复杂:水源、养殖池水、池底污物、附着基板和饵料均发现了病原菌,但病原菌浓度以饵料中最多,附着基板次之,水源中最少。萘啶酸等12种常用抗生素可有效抑制该病原菌的生长。【结论】“化板症”的病原为弧菌Vibriosp.;饵料可能为“化板症”病原的主要来源;萘啶酸等12种常用抗生素可用于该病的防治。
【Objective】 The objective of this study was to identify the pathogen and its origin of the clinging sting plate, and to obtain the treatment of the disease. 【Method】 Pathogen analysis was made on the pathogens of three newly diagnosed hatching farms, and the pathogen was analyzed by artificial backwash infection and morphological, physiological, biochemical and 16S rDNA sequence analysis. The breeding system , Including water, aquaculture water, bottom soil, attached to the substrate and bait for bacteriology analysis, susceptibility testing of pathogenic bacteria. 【Result】 One dominant strain was isolated from all the disease samples. The artificial retroviral infection showed that it had strong pathogenicity to healthy sea cucumber, and the symptoms of the infected sea cucumber were the same. Identified “plate disease ” pathogen Vibrio Vibriosp. A large number of bacteria (> 1 × 10 5 cfu / mL) were detected in all other aquaculture systems, except for bacteria whose concentration was not exceeded (<50 cfu / mL). Sources of pathogens were more complicated: water, aquaculture water, Substrates and bait were found pathogenic bacteria, but the concentration of pathogenic bacteria to feed the most, followed by the substrate, the least water. Nalidixic acid and other commonly used antibiotics 12 can effectively inhibit the growth of the pathogen. 【Conclusion】 The pathogen of “plate disease” is Vibriosp .; Vibriospas may be the main source of “plate disease”; 12 kinds of commonly used antibiotics, such as nalidixic acid, may be used in the prevention and treatment of the disease.