论文部分内容阅读
目的:由文蛤体液中获得一种新的抗肿瘤蛋白。方法:采用电镜技术、流式细胞仪技术、微管蛋白α-tubulin的荧光染色技术、电泳技术,研究了MML作用后细胞凋亡、细胞周期及微管蛋白的变化情况;建立裸鼠肝癌皮下移植瘤模型,观察MML的体内抗肿瘤活性。结果:发现MML可显著抑制肿瘤细胞生长,IC50达到52.2μg·mL-1。作用机制的研究表明MML可以增加肿瘤细胞膜通透性、抑制微管蛋白聚合、使肿瘤细胞停滞在G2/M期;MML对人肝癌BEL-7402移植性肿瘤小鼠具有显著的抗肿瘤作用。结论:文蛤体液蛋白是一种新型的抗肿瘤蛋白,主要通过增加细胞膜的通透性、引起细胞凋亡、抑制微管蛋白聚合产生抗肿瘤作用。
OBJECTIVE: To obtain a new anti-tumor protein from the body fluid of Clam Methods: The apoptosis, cell cycle and the changes of tubulin after MML were studied by electron microscopy, flow cytometry, tubulin α-tubulin fluorescence staining and electrophoresis. Transplanted tumor model to observe the anti-tumor activity of MML in vivo. Results: It was found that MML significantly inhibited tumor cell growth with an IC50 of 52.2 μg · mL -1. Studies on the mechanism of action suggest that MML can increase the membrane permeability of tumor cells, inhibit the polymerization of tubulin, and arrest the tumor cells in the G2 / M phase. MML has a significant anti-tumor effect on human hepatocellular carcinoma BEL-7402 transplanted tumor mice. Conclusion: The clam fluid protein is a new type of anti-tumor protein, mainly through increasing the permeability of cell membrane, causing cell apoptosis and inhibiting tubulin polymerization to produce anti-tumor effect.