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目的探讨全反式维甲酸(atRA)对自发性高血压大鼠(SHR)肾脏血管紧张素1型受体(AT1)及其类似物APJ受体表达的影响。方法采用12wk♂SHR及其同源对照WKY大鼠,经腹腔注射atRA,为期1mon。分别用实时荧光定量PCR与免疫印迹技术测定atRA治疗后SHR肾脏组织AT1和APJ受体的表达情况。结果与WKY对照组相比,SHR肾脏组织中AT1受体mRNA和蛋白水平明显升高,APJ受体表达则出现下调(P均<0·01)。而atRA治疗后SHR肾脏AT1受体表达降低,APJ表达则得到回升,同时伴有血压下降(P均<0·05)。结论长期atRA治疗可调节SHR大鼠肾脏组织中AT1/APJ受体表达的平衡,提示转录调节剂atRA很可能对人类高血压病防治具有一定价值。
Objective To investigate the effects of all-trans retinoic acid (atRA) on APJ receptor expression in renal angiotensin 1 type receptor (AT1) and its analogs in spontaneously hypertensive rats (SHR). Methods 12wk♂SHR and its homologous control WKY rats were injected intraperitoneally with atRA for 1mon. Real-time quantitative PCR and Western blotting were used to detect the expression of AT1 and APJ receptors in SHR kidney tissues after atRA treatment. Results Compared with WKY control group, the mRNA and protein levels of AT1 receptor in SHR were significantly increased and the expression of APJ receptor was down - regulated (all P <0.01). However, the AT1 receptor expression in SHR kidney decreased after atRA treatment, APJ expression was increased, accompanied by decreased blood pressure (all P <0.05). Conclusions Long-term atRA treatment can regulate the balance of AT1 / APJ receptor expression in kidney of SHR rats, suggesting that atRA may play a certain value in the prevention and treatment of human hypertension.