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在自己研制的流式细胞仪上开发出了流式细胞分选装置。分选的方法是给含有目标细胞的液滴充电,使之在静电场中偏转。该装置有以下优点:由于全部电路由同一方波模式振动源驱动,液滴形成过程和充电脉冲产生过程完全同步;独特考虑的分选逻辑电路能识别细胞类型,自动调整充电脉冲宽度,能在保证高分选纯度的前提下获得更高的分选收获率和分选速度;在不应期里出现另一细胞时能予以处理。为了更好地使用分选装置,本文还从仪器角度和样品角度分析讨论了影响分选指标的因素。
Developed in their own flow cytometry flow cytometry sorting device. The method of sorting is to charge the droplets containing the target cells and deflect them in the electrostatic field. The device has the following advantages: Since all the circuits are driven by the same square-wave mode vibration source, the droplet formation process is completely synchronized with the charge pulse generation process; the uniquely considered sorting logic recognizes the cell type and automatically adjusts the charge pulse width, To ensure a high purity of the premise of the premise of access to higher sorting harvest rate and sorting speed; in the refractory period of another cell can be dealt with. In order to make better use of the sorting device, this article also discusses the factors influencing the sorting index from the perspective of equipment and samples.