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利用光控和行为学原理,控制中国对虾在适当时候产卵,在一定的时刻以温度休克和细胞松弛素的方法诱导受精卵发育成为四倍体。作者发展了对虾染色体制备技术,以中肠和触角腺、精巢为材料,从后期幼体直到8~9cm左右次成虾均获得较好的分裂中相。染色体倍性检测结果表明,最好的四倍体诱导成功率达66.7%。共获得10cm左右的实验对虾几千尾。初步观察表明,四倍体中国对虾具有一定的生长优势。
Using the principle of light control and behavior, we control the shrimp in China to lay eggs at the appropriate time and induce the development of fertilized eggs into tetraploids at a certain time by means of temperature shock and cytochalasin. The author developed the chromosome preparation technology of shrimp, using the midgut and the antennal gland, and the testis as materials, and from mid-late larvae to around 8-9 cm secondary shrimp all obtained better split phase. Chromosome ploidy test results showed that the best success rate of tetraploid induction was 66.7%. A total of about 10cm experimental prawn thousands of tail. Preliminary observations indicate that tetraploid P. shrimp has certain growth advantages.