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多样化种质资源的利用对于培育高产和理想农艺性状水稻品种显得非常重要.在本研究中,通过研究继代时间、2,4-D浓度和不同外植体对诱变和分化频率的影响,建立了幼穗为外植体、继代培养时间为3个周期(约75d)以及2,4-D的最佳诱变浓度为4.0mg/L的体细胞诱变育种方法.然后利用该方法诱变杂交水稻亲本株1S和中9B,分别成功选育了优良矮化突变株系SV14S和SV9B,为后续的杂交育种提供了优质种质资源.这些育种成果的取得进一步证实了该水稻体细胞诱变育种方法是一种简单高效的新型育种方法.
The use of diverse germplasm resources is very important for cultivating high yield and ideal agronomic rice varieties.In this study, we studied the effects of subculture time, 2,4-D concentration and different explants on the frequency of mutagenesis and differentiation , The establishment of a young panicle as explants, subculture time of three cycles (about 75d) and 2,4-D of the optimal mutagenesis concentration of 4.0mg / L of somatic mutation breeding method.Then the use of the Methods The hybrid rice parental lines 1S and 9B were mutagenized and the excellent dwarf mutant lines SV14S and SV9B were successfully bred, which provided good germplasm resources for the subsequent cross breeding.These breeding results further confirmed that the rice body Cell mutation breeding method is a simple and efficient new breeding method.