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目的观察非酒精性脂肪性肝病(NAFLD)兔肾组织硫化氢(H2S)、一氧化氮(NO)浓度,探讨H2S、NO在NAFLD肾损害中的作用。方法将40只日本大耳白兔数字法随机分为重度NAFLD组(重度组)、轻度NAFLD组(轻度组)、对照组。重度组予高脂饲料160 g/(兔·d),轻度组予高脂饲料80 g/(兔.d)+普通饲料80 g/(兔.d),对照组予普通饲料160 g/(兔.d)。饲养13周。肾组织匀浆检测NO、H2S浓度。免疫组化染色检测肾组织α-平滑肌肌动蛋白(α-SMA)表达。HE染色,光镜观察肝组织病理学。结果①NO浓度:重度组[(16.24±7.8)μmol/g pro]、轻度组[(22.54±5.4)μmol/g pro]与对照组[(27.32±9.3)μmol/g pro]比较,差异有统计学意义(P<0.01,P<0.05),重度组与轻度组比较差异有统计学意义(P<0.05)。②H2S浓度:重度组、轻度组肾组织H2S与对照组相比明显下降(P<0.01),重度组与轻度组相比差异无统计学意义(P(0.05)。③α-SMA检测:对照组α-SMA存在于血管壁;重度NAFLD组除血管壁外,肾小球系膜区及肾间质均有表达;轻度组较重度组α-SMA表达减轻。④肝脏病理学:重度组肝脏呈重度NAFLD,轻度组呈轻度~中度NAFLD。结论在NAFLD发生、发展过程中肾组织H2S、NO下降,NO、H2S可能参与NAFLD肾损害过程。
Objective To observe the concentration of hydrogen sulfide (H2S) and nitric oxide (NO) in kidneys of nonalcoholic fatty liver disease (NAFLD) and to explore the role of H2S and NO in renal damage of NAFLD. Methods The digital method of 40 Japanese white rabbits were randomly divided into severe NAFLD group (mild group), mild NAFLD group (mild group) and control group. The rats in the control group were treated with 160 g / (rabbit · d) of high fat diet and 80 g / (rabbit.d) of high fat diet and 80 g / (Rabbit .d). Feeding 13 weeks. Kidney homogenate NO and H2S concentrations were measured. The expression of α-smooth muscle actin (α-SMA) in renal tissue was detected by immunohistochemistry. HE staining, light microscopy liver histopathology. Results ① The concentrations of NO in the severe group were (16.24 ± 7.8) μmol / g pro [(22.54 ± 5.4) μmol / g pro], compared with those in the control group [(27.32 ± 9.3) μmol / g pro] (P <0.01, P <0.05). There was significant difference between severe group and mild group (P <0.05). (2) H2S concentration: Compared with the control group, the H2S level in severe group and mild group was significantly decreased (P <0.01), but there was no significant difference between severe group and mild group (P <0.05) .③α-SMA test: In the severe NAFLD group, the expression of α-SMA was observed in both glomerular mesangial area and renal interstitium except in the vessel wall, and the expression of α-SMA in the severe group was relieved.④Histological pathology: severe group The liver showed severe NAFLD and the mild group was mild to moderate NAFLD.Conclusion In the process of NAFLD, the content of H2S and NO in renal tissue decreased, but NO and H2S may be involved in the process of NAFLD.